CONJUGATION TO ANTIBODY

PROCESS

  • Chemical conjugation of payload-linker to the antibody
  • Antibody is provided by the customer (or should be commercially available)
  • Conjugation method: Cys-thiol to maleimide, Lys-amine to NHS-ester etc
  • Feasibility studies to obtain proof of concept (e.g. partial reduction of antibody and then conjugation to obtain first ADC)
  • After feasibility studies, a method for conjugation will be fully developed

ANALYSES

  • Determination of unconjugated payload and unconjugated payload-linker in ADC
  • Spectrophotometric UV/VIS analysis for determination of concentration of ADC
  • Spectrophotometric UV/VIS analysis for determination of mAb-cysteine thiols in the antibody and in the partially reduced antibody (if necessary)
  • Spectrophotometric UV/VIS analysis for determination of DAR in ADC
  • SDS-PAGE analysis of ADC
  • Determination of molecule size distribution of ADC
  • Analysis of ADC (distribution analysis of antibodies loaded with different number of payload molecules)

Our achievements in the complicated field of conjugation is supported by strong R & D and management team.